Case Study: Development of a Scalable LV Production Platform

Developing lentiviral vector production processes that scale reliably across different bioreactor platforms requires more than increasing volume. Cell line behaviour, transfection conditions and engineering parameters all interact and can influence vector yield, consistency and process robustness.

What you’ll learn

In this webinar, Lindsay will present a case study on the development of a suspension-based lentiviral vector production platform using HEK293T cells. The session builds on the concepts introduced in Lodewijk’s previous webinar and provides a step-by-step view of how the process was established and scaled.

You will first see an overview of the full manufacturing workflow before diving into the individual development stages. Lindsay will discuss how adherent HEK293T cells were adapted to suspension culture and how the resulting cell line was established and characterised for stable vector production.

 

Register now

If you work in viral vector process development or manufacturing and want to understand how systematic development strategies can support scalable LV production, this session will provide practical insights.

Register now to secure your place and join the discussion.

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Cell Therapy Development & Manufacturing

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NecstGen is a non-profit CDMO and Centre of Excellence for Cell and Gene Therapy located in a purpose-built GMP facility in Leiden, The Netherlands. Dedicated to the field of Cell and Gene Therapies, we provide expertise and capacity.

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